Effect of Citrus Medica Linn. in Urolithiasis Induced by Ethylene Glycol Model

The Objective of this study is to study the Effect of Citrus medica in urolithiasis induced by Ethylene Glycol model. A number of 24 Male Wistar albino rats (250-300 g) were divided in to 4 groups namely Group I (Normal control), Group II (Model control), Group III (Standard-treated, cystone – 750 mg/kg) and Group IV (Citrus medica – 250 mg/kg). Except group I, Ethylene glycol (0.75% v/v in drinking water) was provided for each group of animals for 28 days study period. After the completion of treatment, various physical parameters (body weight, diuresis, pH), level of urolithiatic promoters (calcium, oxalate, Inorganic phosphate and uric acid) and urolithiatic inhibitors (magnesium and citrate) were measured in 24-h urine, blood samples and kidney homogenate. Antioxidant parameters & renal function tests were carried out as well. Both cystone and extract of Citrus medica showed significant increase in physical parameters and stone forming inhibitors and significant decrease in stone forming promoters. Degree of oxidative stress reduced with cystone and in treatment group. Extract of Citrus medica Linn. showed significant activity against urolithiasis which is due to diuretic and antioxidant activity and its ability to increase inhibitors and decrease promoters levels.